Biochemical characterization of 2 tobacco nicotiana tabacum cultivar xanthi cell lines with different levels of cinnamoyl putrescines

Berlin, J.; Knobloch K H.; Hoefle, G.; Witte, L.

Journal of Natural Products 45(1): 83-87


ISSN/ISBN: 0163-3864
Accession: 004832263

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A p-fluorophenylalanine-resistant tobacco cell line (TX4) and a wild-type culture (TX1) were compared biochemically. The former yielded 6 times more cinnamoyl putrescines than the latter and the main phenolic compounds of both cell lines were identified as caffeoyl-, feruloyl- and p-coumaroyl putrescine. The increased accumulation by TX4 cells was evidently due to increased enzyme activities of related enzymes, phenylalanine ammonia-lyase, trans-cinnamate 4-hydroxylase, p-coumarate-CoA ligase, ornithine and arginine decarboxylases.