Biochemical studies on ginseng saponins part 6 the effect of ginseng saponins on l glutamate dehydrogenase and trans aminase
Joo, C.N.; Oh, J.W.; No, S.J.
Korean Biochemical Journal 9(1): 53-59
The effect of gingseng sapons, major components of Panax ginseng roots, on purified bovine hepatic glutamate dehydrogenase (GLDH) was studied in vitro. The chicken's hepatic mitochondrial glutamate dehydrogenase was previously found to be activated significantly in the presence of saponins; the maximum activity of GLDH was observed when the concentration of the saponins in the assay mixture was 0.1%. The activity of purified bovine hepatic glutamate dehydrogenase in the presence of the saponins was measured and compared with that of control. The activity of GLDH was the highest when the concentration of the saponins in the assay mixture was 10-5 .apprx. 10-6%, the activity being about 1.5 times that of control. In the presence of excess saponins, above 10-4%, in the assay mixture the GLDH was inhibited gradually. The ginseng saponins did not affect ADP activation of GLDH or GTP inhibition of GLDH, signifying that the saponins did not interfere with GLDH regulation of the metabolism involved. The ginseng saponins were realized to activate both the glutamate-pyruvate transaminase and glutamate-oxaloacetate transaminase but the degree of activation was not significant when compared with that of GLDH.