Biochemical studies on ginseng saponins part 8 the effect of ginseng saponins on the oxidation of pyruvate by rats hepatic mitochondrial preparation/
Joo, C.N.; Kim, T.Y.
Korean Biochemical Journal 10(1): 13-22
The effect of ginseng saponin, 1 of the major components of Korean ginseng roots on pyruvate oxidation by rat hepatic mitochondrial preparation was observed to understand the physiological role of the saponin in the TCA [trichloroacetic acid] cycle. The rate of pyruvate oxidation increased gradually as the concentration of saponin in the reaction mixture rose and the maximum oxidation rate was observed at the saponin concentration of 1 .times. 10-4%. An excess amount of saponin, however, inhibited the oxidation. From experiments using radioactive sodium-pyruvate-3-14C, the percent isotope recovered in CO2, absorbed in KOH of the center well of Warburg's manometric reaction vessel during the oxidation of test solution was found to be as much as 2.25 times that of the control; and the pyruvate decrease of the test solution was relatively faster than that of the control. These results also indicated saponin stimulation of pyruvate oxidation. The stimulation of pyruvate oxidation might be the sum of saponin stimulation of individual enzymes of the TCA cycle. It seemed likely that the surface activity of saponin might play a significant role in the enzyme reactions.