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Biochemical studies on ginseng saponins xxv. the effect of ginseng saponin fraction on the biosynthesis of ascorbic acid during germination of soybean glycine max

Bae, S.K.; Kwak, H.S.; Joo, C.N.

Korean Biochemical Journal 19(1): 1-5

1986


ISSN/ISBN: 0368-4881
Accession: 004834205

It was found that ascorbate synthesized during the germination of Glycine max was always much higher than that of control when the soybeans were rinsed in 10-4% ginseng saponin solution at first for 24 hours and the same solution was poured afterwards. Using the homogenate of germinating soybean (2nd day) as enzyme source and glucose containing [U-14C]-glucose as substrate, it was confirmed that the saponin fraction stimulated the ascorbate biosynthesis during germination. From the radioactivity distribution of the intermediates from glucose to ascorbate, it was realized that ascorbate was synthesized from glucose predominantly via galacturonate pathway rather than glucuronate pathway indicating that the glucose was epimerized first to galactose by the action of UDP-glucose epimerase.

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