Biosynthesis of chondroitin sulfate: microsomal proteoglycans

Faltynek, C.R.; Silbert, J.E.

Biochemical and Biophysical Research Communications 83(4): 1502-1508


ISSN/ISBN: 0006-291X
PMID: 697878
DOI: 10.1016/0006-291x(78)91391-8
Accession: 004842548

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A microsomal preparation from chick embryo epiphyseal cartilage was incubated with UDP-[14C]glucuronic acid and UDP-N-acetylgalactosamine to form [14C]chondroitin-labeled proteoglycan. Two [14C]proteoglycan populations were obtained which differed in size, [14C]glycosaminoglycan content and susceptibility to alkali. One population of [14C]proteoglycan appeared near the void volume on Sepharose 2B, while the other population was smaller and similar in size to monomer proteoglycan. The larger [14C]proteoglycan contained long [14C]chondroitin chains added to short primers. These chains were in part resistant to alkali cleavage from protein. The smaller [14C]proteoglycan contained mainly [14C]chondroitin chains of intermediate length added to endogenous chondroitin sulfate. These chains were all susceptible to alkali cleavage from protein. The larger [14C]proteoglycan may represent a precursor proteoglycan present at the site of glycosaminoglycan chain synthesis.