Biosynthesis of cyto kinins by tobacco nicotiana tabacum cultivar xanthi cell cultures

Nishinari, N.; Syono, K.

Plant and Cell Physiology 21(7): 1143-1150


ISSN/ISBN: 0032-0781
Accession: 004842627

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In synchronously cultured tobacco cells (N. tabacum cv. Xanthi), the incorporation of U-14C-adenosine into butanol-soluble cytokinins in vivo was studied. The radioactivity was incorporated into zeatin, ribosylzeatin, isopentenyladenosine and glucosylzeatin after 20 min. The radioactive cytokinins were identified by TLC and high performance liquid chromatography. From the short time course of the incorporation of 14C-adenosine into butanol-soluble cytokinins, the presence of the following biosynthetic pathway in vivo was suggested: adenosine may be converted into isopentenyladenosine and then into zeatin via ribosylzeatin. The biosynthetic pathway of free cytokinins in vivo was compared with that in vitro.