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Biosynthesis of gibberellin a 12 aldehyde gibberellin a 12 and their kaurenoid precursors from carbon 14 mevalonic acid in a cell free system from immature seed of phaseolus coccineus


Phytochemistry (Oxford) 25(1): 97-102
Biosynthesis of gibberellin a 12 aldehyde gibberellin a 12 and their kaurenoid precursors from carbon 14 mevalonic acid in a cell free system from immature seed of phaseolus coccineus
A cell free system capable of gibberellin (GA) biosynthesis has been prepared from immature seed of Phaseolus cocineus. This system converted [14C] mevalonic acid (MVA) to ent-kaurene, ent-kaurenoic acid, ent-Kauradienoic acid, ent-7.alpha.-hydroxykaurenoic acid, ent-6.alpha., 7.alpha.-dihysorxykaurenoic acid, GA12-aldehyde and GA12 ent-[14C] Kaurene was converted to ent-kaurenol, ent-kaurenal, ent-kaurenoic acid and ent-7.alpha.-hydroxykaurenoic acid. All identifications were by GC/MS. The pathway from MVA to GA12-aldehyde in this species thus appears to be the same as that found in cell-free preparations derived from immature seed of other species.


Accession: 004842827



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