+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Bovine coronavirus induced cytopathic expression and plaque formation host cell and virus strain determine trypsin dependence

Bovine coronavirus induced cytopathic expression and plaque formation host cell and virus strain determine trypsin dependence

Journal of Veterinary Medicine Series B 35(1): 48-56

The cytopathic expression (CPE) and plaque formation of different strains of enteropathogenic bovine coronavirus (BCV) were analyzed in a variety of cell types in the presence and absence of trypsin. The cell-adapted BCV-L9, originally isolated by Mebus et al. (1973), and 5 wild-type strains replicated in the HRT-18 line of human rectal tumor cells, inducing cytopathic changes with cell fusion, which was enhanced by trypsin. Strain L9 replicated noncytopathically in D 2 bovine fetal spleen (D 2 BFS) cells, depending absolutely on the presence of trypsin for the activation of cell fusion. D 2 BFS cells were non-permissive for all wild-type BCV strains even in the presence of trypsin. Strain L 9 produced plaques HRT-18 clones, but the ease of plaque formation and plaque morphology were host-cell dependent. Wild-type BCV was also depended on the type of HRT host cell for plaque formation, and a unique plaque morphology with different degrees of trypsin dependence was evident for each strain. The BCV strain and the host cell determined the degree of dependence on trypsin for enhancement of CPE and plaque formation.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 004854897

Download citation: RISBibTeXText

PMID: 3376625

Related references

Structural proteins of bovine coronavirus strain L 9: effects of the host cell and trypsin treatment. Archives of Virology 103(1-2): 35-45, 1988

Plaque formation by african swine fever virus letter cytopathic effects in pig kidney cells uganda virus strain hinde virus strain technique for assay. Nature (London) 219(5153): 524-525, 1968

Enhancement of plaque formation and cell fusion of an enteropathogenic coronavirus by trypsin treatment. Infection and Immunity 31(3): 1214-1222, 1981

Host dependence and virus strain variation of coronavirus mouse hepatitis virus. Abstracts of the Annual Meeting of the American Society for Microbiology 86: 320, 1986

The influence of the host cell and trypsin treatment on bovine coronavirus infectivity. Journal of Veterinary Medicine Series B 35(10): 752-759, 1988

Susceptibility of various cell lines to the cytopathic effect of a strain of bovine papular stomatitis virus. Annales de medecine veterinaire23(5): 333-338, 1979

Antigenic and genomic variations among cytopathic and non-cytopathic strains of bovine enteric coronavirus. Advances in Experimental Medicine and Biology 380: 99, 1995

Patterns of cellular gene expression in cells infected with cytopathic or non-cytopathic bovine viral diarrhea virus. Animal Biotechnology 14(1): 31-49, 2003

Cytopathic effect of Nebraska calf diarrhea virus (Lincoln strain) on secondary bovine kidney cell monolayer. National Institute of Animal Health Quarterly 16(3): 133-134, 1976

Comparative study of various cell lines susceptibility to cytopathic and non-cytopathic strains of Bovine viral diarrhea virus 1 and 2. Acta Virologica 53(4): 287-289, 2009

Expanded host cell tropism and cytopathic properties of feline immunodeficiency virus strain PPR subsequent to passage through interleukin-2-independent T cells. Journal of Virology 74(4): 1854-1863, 2000

Fertility of cows challenged with a cytopathic strain of Bovine Viral Diarrhea virus during an outbreak of spontaneous infection with a noncytopathic strain. Theriogenology 29(2): 441-449, 1988

Influence of genetic and physiological properties of the host cell on the cytopathic expression of herpes simplex virus. Archives of Virology 61(1-2): 127-140, 1979

Cell death induced by cytopathic bovine viral diarrhoea virus is mediated by apoptosis. Journal of General Virology 77: 1677-1681, 1996

Correlation of bovine viral diarrhoea virus induced cytopathic effects with expression of a biotype-specific marker. Archives of Virology. Supplementum 3: 55-66, 1991