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Bovine coronavirus induced cytopathic expression and plaque formation host cell and virus strain determine trypsin dependence



Bovine coronavirus induced cytopathic expression and plaque formation host cell and virus strain determine trypsin dependence



Journal of Veterinary Medicine Series B 35(1): 48-56



The cytopathic expression (CPE) and plaque formation of different strains of enteropathogenic bovine coronavirus (BCV) were analyzed in a variety of cell types in the presence and absence of trypsin. The cell-adapted BCV-L9, originally isolated by Mebus et al. (1973), and 5 wild-type strains replicated in the HRT-18 line of human rectal tumor cells, inducing cytopathic changes with cell fusion, which was enhanced by trypsin. Strain L9 replicated noncytopathically in D 2 bovine fetal spleen (D 2 BFS) cells, depending absolutely on the presence of trypsin for the activation of cell fusion. D 2 BFS cells were non-permissive for all wild-type BCV strains even in the presence of trypsin. Strain L 9 produced plaques HRT-18 clones, but the ease of plaque formation and plaque morphology were host-cell dependent. Wild-type BCV was also depended on the type of HRT host cell for plaque formation, and a unique plaque morphology with different degrees of trypsin dependence was evident for each strain. The BCV strain and the host cell determined the degree of dependence on trypsin for enhancement of CPE and plaque formation.

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Accession: 004854897

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PMID: 3376625


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