Bovine nasal proteo glycans study of the cyanogen bromide fragments from the protein keratan sulfate core and the hyaluronic acid binding region
Perin J P.; Bonnet, F.; Jolles, P.
International Journal of Biological Macromolecules 3(5): 303-310
ISSN/ISBN: 0141-8130 Accession: 004855177
The protein-keratan sulfate core was obtained by chondroitinase treatment of proteoglycan monomers. The hyaluronic acid-binding region of the proteoglycans was prepared by trypsin treatment of the proteoglycans under their associated form. Both preparations were submitted to cyanogen bromide (CNBR) treatment. The fragments were separated by gel filtrations and polyacrylamide gel electrophoreses. The protein-keratan sulfate core gave rise to 2 main classes of fragments: the larger ones apparently arose from the polysaccharide attachment region of the monomers; among the smaller ones 4 appeared to be connected to 1 of the larger fragments by disulfide bridges. The hyaluronic acid-binding region gave rise to fragments in which the electrophoretic mobilities were similar to those of some of the smaller fragments characterized in the CNBR-treated protein-keratan sulfate core. The MW, amino acid and sugar compositions of all the CNBR fragments are reported.