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Breeding and propagating virus free garlic and shallot plants in tissue culture

Breeding and propagating virus free garlic and shallot plants in tissue culture

Doklady Vsesoyuznoi Ordena Lenina i Ordena Trudovogo Krasnogo Znameni Akademii Sel'skokhozyaistvennykh Nauk Imeni V I Lenina (4): 9-11

Elimination of virus infection from shallot and garlic was carried out using cultivation of isolated apical meristem. Addition of sucrose (4.5%) and IBA (1 mg/l) to agarized medium gave good results. Shoots obtained in vitro from the bulb stem and raceme are not capable of eliminating virus infection in the process of regeneration, but may be used successfully for rapid propagation of virus-free material. Regeneration from the stem and raceme proceeds intensively on agarized medium with added sucrose (4.5%) and N-(phenylmethyl)-1H-purin-6-amine (3 mg/ml).

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