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Calcium fluxes across duodenum and colon of spontaneously hypertensive rats effect of 1 25 dihydroxyvitamin d 3



Calcium fluxes across duodenum and colon of spontaneously hypertensive rats effect of 1 25 dihydroxyvitamin d 3



American Journal of Physiology 251(2 Part 2): F278-F282



Calcium absorption by spontaneously hypertensive rats (SHR) was variably reported to be different from normotensive Wistar-Kyoto (WKY) controls. Furthermore, blunted responsiveness to the intestinal effects of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] has also been postulated. To evaluate this hypothesis, calcium fluxes were measured by the Ussing technique across duodenum and descending colon with or without prior 1,25(OH)2D3 treatment. Duodenal mucosal-to-serosal calcium flux (Jm .fwdarw. s) (44.9 vs. 52.4 nmol .cntdot. cm-2 .cntdot. h-1), serosal-to-mucosal flux (Js .fwdarw. m) (25.6 vs. 28.4 nmol .cntdot. cm-2 .cntdot. h-1), and net flux (Jnet) were comparable. 1,25(OH)2D3 increased duodenal Jm .fwdarw. s in both SHR and WKY groups (95.2 and 86.8 nmol .cntdot. cm-2 .cntdot. h-1). Js .fwdarw. m was lower in SHR (26.1 vs. 35.6 nmol .cntdot. cm-2 .cntdot. h-1). Js .fwdarw. m was lower in SHR (26.1 vs. 35.6 nmol .cntdot. cm-2 .cntdot. h-1, P < 0.01), although the tendency for a higher Jnet in SHR (68.6 vs. 51.2 nmoles .cntdot. cm-2 .cntdot. h-1) was statistically insignificant. Short-circuit current was higher in the colon of SHR, both before and after 1,25(OH)2D3, suggesting increased sodium transport. Basal colonic Jnet was virtually zero in both groups but comparably increased by 1,25(OH)2D3 because of stimulation in only Jm .fwdarw. s. Prevention of hypertension by hydralazine since the 4th week of age did not alter the findings compared with the hypertensive SHR, suggesting calcium transport rates were unaffected by hypertension. These data indicate that in vitro, duodenal, and colonic active calcium transport by the SHR is similar to WKY. Their normal responses to 1,25(OH)2D3 do not support the hypothesis of intestinal resistance.

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