Calcium induced formation of chlorophyll b and light harvesting chlorophyll a chlorophyll b protein complex in cucumber cucumis sativus cultivar aonagajibai cotyledons in the dark

Tanaka, A.; Tsuji, H.

Biochimica et Biophysica Acta 680(3): 265-270


ISSN/ISBN: 0005-2728
Accession: 004868214

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Dark-grown cucumber seedlings were exposed to intermittent light (2 min light and 98 min dark) and then cotyledons were incubated with 50 mM CaCl2 in the dark. Chlorophyll (Chl) a was selectively accumulated under intermittent light and Chl b was accumulated during the subsequent dark incubation with CaCl2. The change in Chl-protein complexes during Chl b accumulation induced by CaCl2 in the dark was investigated by SDS-polyacrylamide gel electrophoresis. Chl-protein complex I and free Chl were major Chl-containing bands of the cotyledons intermittently illuminated 10 times. When these cotyledons were incubated with CaCl2 in the dark, the light-harvesting Chl a/b-protein complex was formed. When the number of intermittent illumination periods was extended to 55, small amounts of Chl b and light-harvesting Chl a/b-protein complex were recognized at the end of intermittent light treatment and these 2 pigments were further increased during the subsequent incubation of the cotyledons with CaCl2 in the dark compared to water controls.