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Carbohydrate metabolism in the stem base of orchard grass dactylis glomerata after cutting



Carbohydrate metabolism in the stem base of orchard grass dactylis glomerata after cutting



Grassland Science 28(1): 8-13



Variations in carbohydrate concentration, and phleinase and invertase activities in the stem base of orchard grass (D. glomerata L.) were studied during the regrowth after cutting. Phleinase activity increased drastically during the first 2 days and then kept almost a constant level until it decreased on the 15th day. The phlein concentration was inversely related to this activity, suggesting the involvement of phleinase in the in vivo metabolism of phlein. The concentration of total mono- and oligosaccharides kept almost unchanged, while that of fructose increased rapidly during the first 2 days. Sucrose concentration, which was almost inversely related to the invertase activity, decreased during the first 2 days and recovered to the initial level on the 15th day. Glucose concentration decreased rapidly during the first 5 days and began to increase on the 11th day. Phlein began to accumulate again as sucrose concentration increased at the late stage of regrowth. Apparently, the phleinase hydrolyzes phlein endwise from the elution profiles of phlein on Sephadex G-75 and rapid augmentation of fructose immediately after cutting.

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