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Carbon 13 nmr evidence for 3 slowly inter converting conformations of the di hydro folate reductase nadp folate complex

Carbon 13 nmr evidence for 3 slowly inter converting conformations of the di hydro folate reductase nadp folate complex

Biochemical & Biophysical Research Communications 101(4): 1139-1144

The 3-carboxamido-13C resonance of NADP+ in its complex with Lactobacillus casei dihydrofolate reductase and folate was studied as a function of pH. At low pH a single resonance is observed, while at high pH 2 resonances are observed, neither of which has the same chemical shift as that seen at low pH. The rates of interconversion between the 3 states of the complex represented by these resonances are < 19 s-1 at 11.degree. C.

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