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Carbon 14 labeled carbon di oxide fixation and compartmentation of carbon metabolism in a recombined chloroplast cytoplasm system



Carbon 14 labeled carbon di oxide fixation and compartmentation of carbon metabolism in a recombined chloroplast cytoplasm system



Physiologia Plantarum 46(3): 221-226



The compartmentation of 14CO2 fixation and concomitant metabolism of 14C-labeled products in a recombined system, composed of isolated intact spinach (Spinacia oleracea) chloroplasts and a cytoplasm fraction, was studied. Addition of cytoplasm to chloroplasts fixing 14CO2 increased the label in hexose monophosphates outside the chloroplasts at the expense of excreted dihydroxyacetone phosphate. The label in amino acids was increased inside and outside the chloroplasts. The view that chloroplasts are unable to make 2-oxoacids for amino acid synthesis directly from fixed CO2, but have to cooperate with the cytoplasm and other organelles, was supported. Recombined systems may be useful for studies on the compartmentation of C metabolism in photosynthesizing plant tissues.

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