EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Cell shape and cartilage differentiation of early chick limb bud cells in culture



Cell shape and cartilage differentiation of early chick limb bud cells in culture



Cell Differentiation 11(4): 245-251



The onset of chondrogenesis in the embryonic chick is preceded by a prechondrogenic condensation of the prospective cartilage cells. In culture, the condrogenic phenotype was only expressed by limb mesoderm cells plated at densities above confluence. This led to the proposal that condrogenic differentiation requires immediate histogenic interactions prior to overt chondrogenesis. In both the in vivo and in vitro situations, cells secreting a cartilaginous matrix were rounded in shape. A dependence of phenotypic expression by mature chondrocytes on cell shape was shown. Cells maintained in a rounded configuration by culturing on a semiadhesive substratum (poly(HEMA[hydroxy-ethyl-methacrylate])) synthesized more S-containing extracellular matrix than cells allowed to flatten on normal tissue culture plastic did. Whether there is a similar shape-dependent relationship in the differentiation of chick embryonic mesoblasts into actively secreting chondroblasts was investigated. A rounded cell shape was conducive to the synthesis of a sulfated matrix.

(PDF emailed within 0-6 h: $19.90)

Accession: 004893689

Download citation: RISBibTeXText

PMID: 7116459

DOI: 10.1016/0045-6039(82)90072-0



Related references

Ectodermal inhibition of cartilage differentiation in micromass culture of chick limb bud mesenchyme in relation of gene expression and cell shape. Development (Cambridge) 105(4): 769-778, 1989

Cartilage differentiation in the limb bud of the chick embryo. Ultrastructural observations, culture and grafting experiments. Archives D'anatomie Microscopique et de Morphologie Experimentale 71(4): 241-256, 1982

Differentiation of the cartilage cells in the chick limb bud. Journal of the Anatomical Society of India 19(1): 37, 1970

Epithelial modification of extracellular matrix and the role of cell shape in limb cartilage differentiation. Journal of Cell Biology 97(5 PART 2): 371A, 1983

Muscle cartilage and bone development and differentiation from chick limb mesenchymal cells. Ede, D A , J R Hinchliffe And Michael Balls (Ed ) British Society For Developmental Biology Symposium 3 Vertebrate Limb And Somite Morphogenesis Glasgow, Scotland, Sept 1-4, 1976 Cambridge University Press: New York, N Y , Usa; Cambridge, England Isbn 0-521-21552-8 199-213, 1977

c-Myc overexpression increases cell size and impairs cartilage differentiation during chick limb development. Cell Growth & Differentiation 13(4): 185-193, 2002

Requirement of vitamin C for cartilage calcification in a differentiating chick limb-bud mesenchymal cell culture. Bone 12(4): 277-282, 1991

Benzamide on chondrocytic differentiation in chick limb bud cell culture. Journal of Embryology and Experimental Morphology 85: 163-175, 1985

Cell populations synthesizing cartilage proteoglycan core protein in the early chick limb bud. Biochemical & Biophysical Research Communications 174(2): 688-695, 1991

Interrelationship between poly (ADP-Rib) synthesis, intracellular NAD levels, and muscle or cartilage differentiation from mesodermal cells of embryonic chick limb. Proceedings of the National Academy of Sciences of the United States of America 72(5): 1852-1857, 1975

Studies on growth and differentiation of cartilage cells from creeper chick embryos in culture. Japanese Journal of Genetics 48(3): 197-205, 1973

An immunological study of cartilage differentiation in cultures of chick limb bud cells: influence of a tumor promoter (TPA) on chondrogenesis and on extracellular matrix formation. Progress in Clinical and Biological Research 110 Pt B: 159-166, 1982

Interrelationship between poly adp ribose synthesis intra cellular nad levels and muscle or cartilage differentiation from mesodermal cells of embryonic chick limb. Proceedings of the National Academy of Sciences of the United States of America 72(5): 1852-1857, 1975