Changes in endogenous cell surface galactosyltransferase activity during in vitro limb bud chondrogenesis

Shur, B.D.; Vogler, M.; Kosher, R.A.

Experimental Cell Research 137(1): 229-237

1982


ISSN/ISBN: 0014-4827
PMID: 6799308
Accession: 004907507

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Abstract
When the subridge mesoderm of the embryonic chick limb bud is cultured in the absence of the apical ectodermal ridge and adjacent ectoderm, the cells rapidly progress through the various stages of chondrogenesis. During the 1st day of culture, the cells initiate condensation and during subsequent days, deposit a cartilage matrix. Here, it was shown that early in the 1st day there is a progressive 2-fold increase in cell surface galactosyltransferase activity towards endogenous acceptors. Later in the 1st day, although the cells are still in condensation, endogenous galactosyltransferase activity decreased, suggesting in situ galactosylation of surface acceptors. During subsequent development, when cartilage matrix is being deposited, surface galactosyltransferase activity remains low. Al controls were performed to insure cell surface localization of enzyme activity. Two other surface glycosyltransferases show very low levels of activity, which do not change significantly during culture. During cellular condensation, an interaction between surface galactosyltransferases and acceptors on adjacent cells may occur; this interaction may be causally related to subsequent chondrogenic differentiation.