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Changes in the distribution of the 34 kilodalton tyrosine kinase substrate during differentiation and maturation of chicken tissues/


, : Changes in the distribution of the 34 kilodalton tyrosine kinase substrate during differentiation and maturation of chicken tissues/. Journal of Cell Biology 98(2): 473-486

The distribution of the 34-kilodalton (34-kD) tyrosine kinase substrate was examined in tissues of adult and embryonic chicken using both a mouse monoclonal antibody and a rabbit polyclonal antibody raised against the affinity purified 34 kD protein. The localization was analyzed by immunoblotting of tissue extracts, by immunofluorescence staining of frozen tissue sections and by staining sections of paraffin-embedded organs by the peroxidase anti-peroxidase method. The 34-kD protein was present in a variety of cells, including epithelial cells of the skin, gastrointestinal and respiratory tracts, as well as in fibroblasts and chondrocytes of connective tissue and mature cartilage, and endothelial cells of blood vessels. The 34-kD protein was also found in subpopulations of cells in thymus, spleen, bone marrow and bursa. The protein was not detected in cardiac, skeletal or smooth muscle cells, nor in epithelial cells of liver, kidney, pancreas and several other glands. Although most neuronal cells did not contain the 34-kD protein, some localized brain regions did contain detectable amounts of this protein. The 34-kD protein was not detected in actively dividing cells of a number of tissues. Changes in the distribution of the 34-kD protein were observed during the differentiation or maturation of cells in several tissues including epithelial cells of the skin and gastrointestinal tract, fibroblasts of connective tissue and chondroblasts.

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Related references

Greenberg, M.E.; Brackenbury, R.; Edelman, G.M., 1984: Changes in the distribution of the 34-kdalton tyrosine kinase substrate during differentiation and maturation of chicken tissues. We examined the distribution of the 34-kilodalton (34-kD) tyrosine kinase substrate in tissues of adult and embryonic chicken using both a mouse monoclonal antibody and a rabbit polyclonal antibody raised against the affinity purified 34 kD protei...

Glenney J., 1986: Phospholipid dependent calcium binding by the 36 kilodalton tyrosine kinase substrate calpactin and its 33 kilodalton core. A 36-kDa protein, which is a component of the membrane skeleton, has been shown to co-localize with spectrin in addition to serving as a major substrate for tyrosine-protein kinases. This protein, which will be referred to as calpactin (for calciu...

Xu, Y.; Singer, M.A.; Lindquist, S., 1999: Maturation of the tyrosine kinase c-src as a kinase and as a substrate depends on the molecular chaperone Hsp90. Although Hsp90 displays general chaperone activity in vitro, few substrates of the chaperone have been identified in vivo, and the characteristics that render these substrates dependent on Hsp90 remain elusive. To investigate this issue, we exploi...

Xu, Y.S.nger, M.; Lindquist, S., 1999: Maturation of the tyrosine kinase c-src as a kinase and as a substrate depends on the molecular chaperone Hsp90. Although Hsp90 displays general chaperone activity in vitro, few substrates of the chaperone have been identified in vivo, and the characteristics that render these substrates dependent on Hsp90 remain elusive. To investigate this issue, we exploi...

Sefton B.M.; Hunter T.; Cooper J.A., 1983: Some lymphoid cell lines transformed by abelson murine leukemia virus lack a major 36 kilodalton tyrosine protein kinase substrate. Mouse fibroblasts transformed by Abelson murine leukemia virus differ from normal fibroblasts in that they contain several cellular proteins, including 1 of 29 and 1 of 36 kilodaltons [kd], which are phosphorylated at tyrosine residues. Since it h...

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Gould, K.L.; Cooper, J.A.; Hunter, T., 1984: The 46,000-dalton tyrosine protein kinase substrate is widespread, whereas the 36,000-dalton substrate is only expressed at high levels in certain rodent tissues. Proteins of molecular mass 46,000 (p46) and 34,000-39,000 (p36) daltons are phosphorylated at tyrosine in Rous sarcoma virus-transformed chicken and mouse fibroblasts. p46 has recently been identified as an isozyme of enolase but the function of p...

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