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Changes in the synthesis of rna and protein during tracheary element differentiation in single cells isolated from the mesophyll of zinnia elegans


Changes in the synthesis of rna and protein during tracheary element differentiation in single cells isolated from the mesophyll of zinnia elegans



Plant and Cell Physiology 24(4): 603-614



ISSN/ISBN: 0032-0781

DOI: 10.1093/oxfordjournals.pcp.a076556

Cycloheximide (CH) prevented tracheary element (TE) differentiation and cell division in a culture of single cells isolated from the mesophyll of Z. elegans at the concentrations which inhibited incorporation of [14C]-leucine into protein. When the cells were pulse-treated with this inhibitor for 12 h at various times of culture, TE formation was inhibited most strongly by the treatments made between 24 and 60 h of culture. Incorporation of [14C]-leucine into protein showed a high level during this period. The inhibitory effect of actinoomycin D (Act-D) on TE differentiation was also marked when it was administered from 24-60 h of culture when incorporation of [14C]-uridine into nucleic acid was at a higher level. Apparently, RNA and protein syntheses are prerequisites for cytodifferentiation to TE and the syntheses between 24 and 60 h of culture are closely associated with cytodifferentiation. Studies of qualitative changes in proteins using 2-dimensional electrophoresis revealed that .apprx. 400 polypeptides extracted from [35S]-methionine-labeled cells could be reproducibly resolved and that most of them were synthesized in both differentiating and nondifferentiating cells. During TE differentiation, however, the synthesis of 2 polypeptides was shut off and 2 other polypeptides were newly synthesized between 48 and 60 h of culture, preceding the morphological changes. The relationship between TE differentiation and the synthesis of RNA and protein is discussed.

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Accession: 004915977

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