Section 5
Chapter 4,917

Changes of 3 4 di hydroxy benzoic acid and 4 hydroxy benzoic acid in nicotiana tabacum cell suspension culture

Hrubcova, M.; Cvikrova, M.; Pospisil, F.

Biologia Plantarum 23(4): 318-320


ISSN/ISBN: 0006-3134
Accession: 004916863

The optimum conditions for cell suspension culture experiments were characterized by respiration, p-phenylenediamine oxidase and growth of cell suspension of N. tabacum for 15 days in stationary culture. Change of 3,4-dihydroxybenzoic and 4-hydroxybenzoic acids both present at 10-4 M in culture medium were studied in short term experiments. An oxidation product of 3,4-dihydroxybenzoic acid characterized by an absorption maximum at 280 nm was isolated after 1 h of incubation. The 4-hydroxybenzoic acid did not change when incubated for 4 h under the same conditions.

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