Characteristics of histamine sensitive adenylate cyclase in the rat gastric mucosa cells

Ivashkin, V.T.; Ageeva, O.G.; Konicheva, T.L.; Chesnokova, L.S.; Vasil'eva V.Yu

Fiziologicheskii Zhurnal SSSR Imeni I M Sechenova 72(11): 1552-1558


ISSN/ISBN: 0015-329X
Accession: 004920789

Download citation:  

Article/Abstract emailed within 1 workday
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Effects exerted by specific modifiers and ions on the adenylate cyclase activity of coarse membrane fractions of cells isolated from the rat gastric mucosa, were investigated. Fluoride ions cause 7-8-fold activation of the enzyme affecting neither the enzyme's affinity towards Mg2+-ATP, nor the cooperativeness of substrate binding. Cations Mg2+ and Mn2+ produce activating while Ca2+ inhibiting effects. GTP (10-5 M) enhances histamine activation of the enzyme and reduces maximal stimulating concentrations of histamine. GTP and Gpp(NH)p exert activating action on the enzyme causing 2- and 5-fold activation, resp. Adenosine suppresses the enzyme activity, the effect being potentiated by GTP. The plausible role of N-protein in the regulation of the enzyme activity is discussed.