Characterization of intraspecific somatic hybrids of carrot daucus carota obtained by fusion of iodo acetate inactivated azetidine 2 carboxylic acid resistant and sensitive protoplasts

Cella, R.; Carbonera, D.; Iadarola, P.

Zeitschrift fuer Pflanzenphysiologie 112(5): 449-458


Accession: 004929275

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Appearance of A2CA-resistant colonies from a mixed population of iodoacetate-inactivated A2CA-resistant and sensitive carrot protoplasts was dependent upon fushion. No colonies arose from controls in which mixed unfused (or homologously fused) population of parental protoplasts were plated in order to rule out cross-feeding. Suspension cultures obtained from resistant colonies were tested for level of resistance to A2CA, uptake of the analogue and endocellular content of free proline. All these parameters were found to be intermediate between those of parental lines. Unexpectedly, the chromosome number of hybrid lines was very close to that of the sensitive parental line suggesting that extensive chromosome loss had occurred in fusion products. These experiments show that resistance to A2CA due to proline overproduction behaves as a semidominant trait.