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Characterization of macrophage activation factor a lymphokine that causes macrophages to become cyto toxic for tumor cells



Characterization of macrophage activation factor a lymphokine that causes macrophages to become cyto toxic for tumor cells



Cellular Immunology 41(2): 347-357



Macrophage activation factor (MAF) was isolated from PPD[purified tuberculin]stimulated, BCG-immune mouse spleen cell culture fluids. In 9 gel filtration runs on Sephadex G-100 or G-200, MAF was eluted in a single peak corresponding to a MW of 55,000 .+-. 1600. Recovery of activity was about 65%. Since the relative concentration curve of eluted MAF was wider than that of a single protein, MAF activity may be due to more than one protein with similar MW. 7his possiblility was strengthened by a broad elution range on DEAE cellulose chromatography, from a specific conductance of 3.5-8.5 mmho/cm, at pH 7.9. MAF was labile at pH 4 and 10, and was destroyed by proteolytic enzymes. By heating at 56.degree. C for 30 min, 80% was destroyed. In affinity chromatography experiments, MAF did not bind to Con [concanavalin] A Sepharose. It was bound to insolubilized Cibacron-blue and was eluted by an increase in ionic strength.

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Accession: 004929598

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PMID: 31981


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