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Chapter 4,931

Characterization of nucleolar antigens of normal rat liver, regenerating rat liver, and Novikoff ascites hepatoma cells following in vitro translation of polyadenylic acid-containing messenger RNA

Satoh, K.; Busch, H.

Cancer Research 43(5): 2143-2149

1983


ISSN/ISBN: 0008-5472
PMID: 6831440
Accession: 004930366

Nucelolar antigens of normal rat liver, regenerating liver and Novikoff ascites hepatoma cells were translated in vitro from polyadenylic acid-containing mRNA isolated from the respective tissues and immunoprecipitated with specific antinucleolar antibodies and Protein A. By 2-dimensional gel electrophoresis of the translation proudcts, 5 antigens were detected in normal rat liver. The antigens detected in 18-h regenerating rat liver were the same as those of normal rat liver when immunoprecipitated with the anti-liver nucleolar antibodies. In the Novikoff tumor, 11 antigens were detected with anti-Novikoff nucleolar antibodies. Of these, 2 were not found in either normal or regenerating liver. Four major antigens were detectable in both Novikoff hepatoma and regenerating liver mRNA translation products with anti-Novikoff nucleolar antibodies. Two antigens were found in normal and regenerating liver that were not found in Novikoff hepatoma. These immunoprecipitation analyses provide further evidence that some nucleolar antigens are present in Novikoff hepatoma that are not found in either normal or regenerating rat liver.

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