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Characterization of pyrimidine deoxyribonucleoside kinase (thymidine kinase) and thymidylate kinase as a multifunctional enzyme in cells transformed by herpes simplex virus type 1 and in cells infected with mutant strains of herpes simplex virus

Chen, M.S.; Summers, W.P.; Walker, J.; Summers, W.C.; Prusoff, W.H.

Journal of Virology 30(3): 942-945

1979

ISSN/ISBN: 0022-538X
PMID: 225551
Accession: 004931102
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Pyrimidine deoxyribonucleoside kinase (thymidine kinase [TK]) was purified from 2 herpes simplex virus type 1 (HSV-1)-transformed TK-deficient mouse (LMTK-) cell lines and from LMTK- cells infected with HSV-1 mutant viruses coding for variant TK enzymes. These preparations exhibited normal or variant virus-induced thymidylate kinase activities correlating with their relative TK activities. Neither virus-induced activity was detected in LMTK- cells infected with an HSV-1 TK-deficient mutant. HSV-1 thymidylate kinase activity and TK activity are mediated by the same protein.

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