+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Characterization of rennet preparations produced by genetic engineering in comparison to calf rennet

Characterization of rennet preparations produced by genetic engineering in comparison to calf rennet

Milchwissenschaft 42(12): 787-789

ISSN/ISBN: 0026-3788

For a comparative analysis of calf rennet and rennet preparations produced by genetic engineering different methods for the purity and identity test as well as for measuring the proteolytic activity are described. The anion exchange column chromatography was used for the pre-purification of the chymosin and the pepsin fraction, resp. By the fine structure of UV spectra in derivative spectroscopy (2nd and 4th derivative) it was possible to differentiate pepsin and chymosin. The immunochemical detection of chymosin and pepsin was done by radial agar gel double diffusion. By its high resolution the ultrathin-layer isoelectric focusing (UDIEF) was adapted for the identification of genetic variants of chymosin. For a differentiation of the proteolytic enzymes from the concomitant rennet proteins in the electropherogram the substrate gel technique was used. A skim-milk powder agarose diffusion test served as a micromethod for the quantitative determination of the proteolytic enzymes. The proteolytic activity of rennet enzymes could be characterized by renneting strength determination and by the HPLC peptide pattern after the proteolysis of .alpha.S1-casein. The content of phosphotungstic acid soluble amino-N which could be determined by o-phthaldialdehyde (OPA) in aqueous cheese extracts was an appropriate measure of proteolysis in cheese.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 004931299

Download citation: RISBibTeXText

Related references

Characterization of rennet preparations produced by genetic engineering in comparison to calf rennet 2. results. Milchwissenschaft 43(2): 71-75, 1988

Physicochemical, molecular and immunological characterization of camel calf rennet: a comparison with buffalo rennet. Journal of Dairy Research 67(1): 73-81, 2000

Extraction of camel rennet and its comparison with calf rennet extract. Milchwissenschaft 48(6): 322-325, 1993

Characterization of mixtures of calf rennet and rennet substitutes. Nelh. Milk Dairy J 23(1): 55-70, 1969

Characteristics of miniature Cheddar-type cheese made by microbial rennet from Bacillus amyloliquefaciens: a comparison with commercial calf rennet. Journal of Food Science 79(2): M214-M221, 2014

Characterization of rennet-induced gels using calf rennet processed by high pressure homogenization: Effects on proteolysis, whey separation, rheological properties and microstructure. Innovative Food Science and Emerging Technologies 26: 517-524, 2014

Yield, body and texture of Cheddar cheese made from milk, 1.5x, or 4.8x UF retentates and set with varying amounts of calf rennet or a 50:50 mixture of calf rennet-porcine pepsin. Journal of Dairy Science 72(Suppl 1): 131-132, 1989

Manufacture of Bulgarian White cheese, using calf rennet and a calf rennet/pepsin mixture. Industria Latte 6(4): 217-29, 1970

Comparison of fermentation produced chymosin and calf rennet in cheddar cheese. Australian Journal of Dairy Technology 45(2): 53-59, 1990

A simple method for distinguishing calf rennet from micro-bial rennet substitutes. Milckwissenschrft 26(11): 665-668, 1971

Experiments comparing microbial rennet with calf rennet in Taleggio cheese production. Industria del Latte 12(1): 43-44, 1976

Contribution of calf rennet and noury rennet proteases to proteolysis in edam cheese during ripening. Asian Journal of Dairy Research 4(4): 203-208, 1985

Bitterness in Ras cheese made with soluble and immobilized calf rennet and Mucor miehei rennet. Egyptian Journal of Dairy Science 17(2): 207-216, 1989

A comparative study of the physico-chemical properties of fistulated calf rennet and commercial rennet. Milchwissenschaft 32(5): 286-290, 1977

Commercial trials using 'Rennilase' microbial rennet as a substitute for calf rennet in cheese manufacture. Dairy Products 5(1): 9-11, 1977