EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Characterization of sodium potassium atpase ec 3.6.1.3 liposomes 1. effect of enzyme concentration and modification on liposome size intra membrane particle formation and sodium potassium transport


Biochimica et Biophysica Acta 773(2): 253-261
Characterization of sodium potassium atpase ec 3.6.1.3 liposomes 1. effect of enzyme concentration and modification on liposome size intra membrane particle formation and sodium potassium transport
Rabbit renal (Na+ + K+)-ATPase was purified and incorporated into phosphatidylcholine liposomes. Freeze-fracture analysis of the reconstitutedsystem reveals intramembrane particles formed by (Na+ + K+)-ATPase molecules which are randomly distributed on concave and convex fracture faces. The reconstituted (Na+ + K+)-ATPase performs active Na+,K+-transport. The distribution of particles as well as the rate of active transport are directly proportional to the (Na+ + K+)-ATPase protein concentration used for reconstitution, while the total amount of Na and K exchanged by ATP per volume vesicle suspension reaches maximum when each vesicle contains on the average > 2 particles. (Na+ + K+)-ATPase pretreated with ouabain or vanadate yields the same particle density and vesicle size as control enzyme. However, detergent-denatured enzyme loses its ability to form intramembrane particles or to increase the vesicle size indicating that the lipids surrounding the protein part of the molecule are essential for the reconstitution process. The vesicle diameter increases as a function of the number of particles per vesicle. Histograms of the size distribution become wider with increasing intramembrane particle density and tend to show > 1 maximum.


Accession: 004931646



Related references

Characterization of sodium potassium atpase liposomes 2. effect of alpha subunit digestion on intra membrane particle formation and sodium potassium transport. Biochimica et Biophysica Acta 773(2): 262-270, 1984

Characterization of (Na+ + K+)-ATPase liposomes. I. Effect of enzyme concentration and modification on liposome size, intramembrane particle formation and Na+,K+-transport. Biochimica et Biophysica Acta 773(2): 253-261, 1984

Purification and characterization of sodium potassium atpase part 7 tryptic degradation of the sodium form of the enzyme protein resulting in selective modification of dephosphorylation reactions of the sodium potassium atpase. Biochimica et Biophysica Acta 507(1): 8-16, 1978

Purification and characterization of sodium ion plus potassium ion stimulated atpase part 8 altered sodium ion to potassium ion transport ratio in vesicles reconstituted with purified sodium ion plus potassium ion stimulated atpase that has been selectively modified with trypsin in presence of sodium chloride. Biochimica et Biophysica Acta 555(3): 485-492, 1979

Preparation of sodium potassium atpase ec 3.6.1.3 containing liposomes with predictable transport properties by a procedure relating the sodium potassium transport capacity to the atpase activity. Journal of Biochemical and Biophysical Methods 5(6): 299-306, 1981

Immunochemical studies for the characterization of purified sodium potassium atpase and its subunits with a special reference of their effect on monovalent cation transport in reconstituted sodium potassium atpase vesicles. Korean Journal of Pharmacology 26(1): 35-50, 1990

Peripheral effects of thyroid hormones alteration of intra cellular sodium concentration sodium potassium atpase and sodium lithium counter transport activity. Acta Endocrinologica Supplementum 105(264): 50`, 1984

Sodium potassium atpase mediated cation pumping in cultured rat hepatocytes rapid modulation by alanine and tauro cholate transport and characterization of its relationship to intra cellular sodium concentration/. Journal of Biological Chemistry 258(21): 12912-12919, 1983

Parameters of reconstituted sodium and potassium transport in liposomes in which purified sodium potassium atpase is incorporated by freeze thaw sonication. Skou, J C And J G Norby (Ed ) Na,k-Atpase: Structure And Kinetics; 2nd International Conference on Properties And Functions Of Na,k-Atpase, Sonderborg, Denmark, Sept 17-21, 1978 Xii+549p Academic Press, Inc : New York, N Y , Usa Illus P47-68, 1979

Purification and characterization of sodium ion and potassium ion dependent atpase part 6 differential tryptic modification of catalytic functions of the purified enzyme in presence of sodium chloride and potassium chloride. Biochimica et Biophysica Acta 466(1): 97-108, 1977