+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Chimerism in lycopersicon peruvianum plants regenerated from in vitro cultures of anthers and stem internodes



Chimerism in lycopersicon peruvianum plants regenerated from in vitro cultures of anthers and stem internodes



Zeitschrift fuer Pflanzenzuechtung 76(4): 299-319



In vitro cultures of anthers and stem internodes of different self-incompatible genotypes of L. peruvianum were used to obtain plant regeneration, and gain information on the possible application of the in vitro culture techniques to researches on self-incompatibility. From a total of 8 diploid and 2 autotetraploid genotypes used, regeneration was obtained from anther cultures in 2 diploid genotypes and from stem-explants in 3 diploid genotypes. Cytological analyses on 82 regenerated plants revealed the presence of diploid, tetraploid and cytochimeric (diplo-tetraploid) plants. Determination of the ploidy of the histogenetic layers L1, L2 and L3 in the cytochimeras showed that part were periclinal and part mericlinal. DNA cytophotometry of the nuclei isolated from the calli derived from anther cultures demonstrated the occurrence of different ploidy levels. The possible origin of in vitro regenerated plants including the cytochimeras and the influence of culture conditions (composition of the medium and age of culture) are discussed in the light of the results obtained in the present study.

(PDF emailed within 1 workday: $29.90)

Accession: 004943735

Download citation: RISBibTeXText


Related references

Plant regeneration from in vitro cultures of anthers and stem internodes in an interspecific hybrid lycopersicon esculentum x lycopersicon peruvianum and cyto genetic analysis of the regenerated plants. Plant Science Letters 20(2): 157-166, 1980

Plant regeneration from in vitro cultures of stem internodes in self-incompatible triploid Lycopersicon peruvianum Mill. and cytogenetic analysis of regenerated plants. Plant Science Letters 22(3): 197-204, 1981

Genetic analyses of plants regenerated from in vitro culture of anthers and stem-internodes in Lycopersicum peruvianum. Incompatibility Newsletter (6): 33-34, 1975

In vitro culture of anthers and stem internodes of Lycopersicum peruvianum: nuclear DNA determination in calli and cytological analysis of regenerated plants. Zeitschrift fur Pflanzenphysiologie 82(5): 377-388, 1977

In vitro culture of the anthers and internodes of Lycopersicon peruvianum. Informatore botanico italiano: 8 (1) 50-54, 1976

Genetic instability at the s locus of lycopersicon peruvianum plants regenerated from in vitro culture of anthers generation of new s specificities and s allele reversions. Heredity 49(3): 319-330, 1982

Origin and genetic analysis of plants regenerated in vitro from periclinal chimeras of lycopersicon peruvianum. Zeitschrift fuer Pflanzenzuechtung 77(2): 112-120, 1976

Dihaploid plants regenerated from tomato anthers in vitro Lycopersicon esculentum. Plant tissue culture 1982: proceedings 5th International Congress of Plant Tissue and Cell Culture held at Tokyo and Lake Yamanake Japan July 11-16-1982 edited by Akio Fujiwara: 550, 1982

Ultrastructure of the exine and of the tapetal film of the anthers in tomatoes (Lycopersicon esculentum Mill., Lycopersicon peruvianum Mill., Lycopersicon hirsutum Humb. et Bonp.). Trudy po prikladnoi botanike genetike i selektsii0(2): 16-24, 1977

Ontogenetic origin of in vitro - regenerated plants in Lycopersicum peruvianum. Incompatibility Newsletter (6): 76-79, 1975

Cross compatibility between the cultivated tomato lycopersicon esculentum and the wild species lycopersicon peruvianum lycopersicon chilense assessed by ovule culture in vitro. Japanese Journal of Breeding 41(2): 223-230, 1991

In vitro culture conditions, regeneration of plants with different ploidies and chimerism, and cytophotometric analyses in Lycoperiscum peruvianum. Incompatibility Newsletter (6): 31-32, 1975

Growth cycle of suspension cultures of Lycopersicon esculentum and Lycopersicon peruvianum Tomatoes. Biochemie und Physiologie der Pflanzen77(6): 483-499, 1982

Growth cycle of suspension cultures of lycopersicon esculentum cultivar lukullus and lycopersicon peruvianum. Biochemie und Physiologie der Pflanzen (BPP) 177(6): 483-499, 1982

Functional cybrid plants of Lycopersicon peruvianum var 'dentatum ' with chloroplasts of Lycopersicon esculentum. Plant Cell Reports 19(6): 588-597, 2019