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Chlorophyll fluorescence spectra of green bean leaves

Chlorophyll fluorescence spectra of green bean leaves

Journal of Plant Physiology 129(1-2): 137-148

By use of an optical multichannel analyzer (OMA) the full chlorophyll fluorescence spectra of green bean leaves (Phaseolus vulgaris L.) were first registered during the induction period of the in vivo chlorophyll fluorescence (Kautsky effect). The chlorophyll fluorescence spectra of leaves exhibit maxima near 690 nm or 735 nm or at both wavelengths depending on the chlorophyll content. The laser-induced fluorescence spectra were measured in 20 ms time periods during the fast fluorescence rise and in 200 ms periods during the slow fluorescence decrease, which proceeds in a range of minutes. Greater changes in the shape of the spectra-due to the chlorophyll content of the sample-and minor changes-due to the phase of the induction period-were determined by measuring the fluorescence kinetics simultaneously at 690 and 735 nm.

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Accession: 004945472

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DOI: 10.1016/s0176-1617(87)80110-4

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