+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Cholinergic regulation of protein III phosphorylation in bovine adrenal chromaffin cells



Cholinergic regulation of protein III phosphorylation in bovine adrenal chromaffin cells



Journal of Neuroscience 8(9): 3233-3239



Protein IIIa (Mr approximately 74,000) and protein IIIb (Mr approximately 55,000), referred to collectively as protein III, are synaptic vesicle-associated phosphoproteins found in all regions of the rat nervous system and in the rat adrenal medulla. In the present study, the presence of protein III and the regulation of its phosphorylation were examined in chromaffin cells isolated from bovine adrenal medullae. Protein III was present in chromaffin cells isolated and purified from bovine adrenal medullae. The levels of protein III were moderately enriched in purified chromaffin cells compared with whole adrenal medullae. Preincubation of chromaffin cells with 32PO4 led to the endogenous phosphorylation of protein III, and phosphopeptide maps of chromaffin cell protein III were similar to those of protein III from bovine cerebral cortex. Treatment of the chromaffin cells with ACh produced calcium-dependent increases in both the phosphorylation of protein III and the release of 3H-norepinephrine. These effects of ACh were mimicked by nicotine but not by muscarine. Other secretagogues (elevated K+, veratridine, Ba2+) also increased both the phosphorylation of protein III and the release of 3H-norepinephrine. However, detailed characterization of the secretagogue-induced increases in protein III phosphorylation and 3H-norepinephrine secretion suggested that protein III phosphorylation was more directly associated with an increase in intracellular calcium than with secretion per se.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 004948533

Download citation: RISBibTeXText

PMID: 3139846


Related references

Cholinergic regulation of protein phosphorylation in bovine adrenal chromaffin cells. Proceedings of the National Academy of Sciences of the United States of America 85(5): 1677-1681, 1988

Cholinergic regulation of protein Iii phosphorylation in bovine adrenal chromaffin cells. The Journal of Neuroscience 8(9): 3233-3239, 1988

Cholinergic receptor-mediated phosphorylation and activation of tyrosine hydroxylase in cultured bovine adrenal chromaffin cells. Journal of Neurochemistry 46(2): 610-622, 1986

Nicotinic cholinergic regulation of tetrahydrobiopterin levels in bovine adrenal chromaffin cells. Advances in Experimental Medicine and Biology 338: 235-238, 1993

Na+/Ca2+ exchanger in bovine adrenal chromaffin cells - possible regulation by phosphorylation. Society for Neuroscience Abstracts 24(1-2): 568, 1998

Protein phosphorylation in bovine adrenal medullary chromaffin cells: histamine-stimulated phosphorylation of tyrosine hydroxylase. Journal of Neurochemistry 59(1): 164-174, 1992

Cholinergic regulation of protein iii phosphorylation in isolated chromaffin cells. Society for Neuroscience Abstracts 10(1): 196, 1984

Tyrosine hydroxylase gene expression in bovine adrenal chromaffin cells regulation by cholinergic receptor stimulation. Society for Neuroscience Abstracts 16(2): 960, 1990

Histamine induced protein phosphorylation in bovine adrenal chromaffin cells. Journal of Neurochemistry 52(Suppl.): S88, 1989

Tyrosine hydroxylase phosphorylation in digitonin-permeabilized bovine adrenal chromaffin cells: The effects of protein kinase and phosphatase inhibitors on Ser19 and Ser40 phosphorylation. Journal of Neurochemistry 69(6): 2387-2396, 1997

Nicotinic cholinergic regulation of tyrosine hydroxylase gene expression and catecholamine synthesis in isolated bovine adrenal chromaffin cells. Journal Of Neurochemistry. 59(6): 2285-2296, 1992

Phorbol dibutyrate increases protein iii phosphorylation in bovine adrenal chromaffin cells. Society for Neuroscience Abstracts 11(2): 846, 1985

Evidence that phosphorylation events and protein synthesis are important in nicotinic receptor turnover in bovine adrenal chromaffin cells. Society for Neuroscience Abstracts 22(1-3): 1523, 1996

Involvement of protein kinase C in the regulation of Na+/Ca2+ exchanger in bovine adrenal chromaffin cells. Clinical and Experimental Pharmacology and Physiology 36(7): 717-723, 2009

G protein regulation of the BK calcium-activated potassium channel in bovine adrenal chromaffin cells. Society for Neuroscience Abstracts 20(1-2): 726, 1994