Chromosomal variability in tissue cultures and regenerated plants of Hordeum

Orton, T.J.

Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 56(3): 101-112

1980


ISSN/ISBN: 0040-5752
PMID: 24305759
DOI: 10.1007/bf00265080
Accession: 004951690

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Abstract
Spontaneous polyploidy, aneuploidy, and chromosomal rearrangements were observed in callus and suspension cultures of Hordeum vulgare, H. jubatum, and their interspecific hybrid. The extent to which each class of chromosomal variability was present in a culture depended upon differentiated state, age, and history. Cytological and isozymic analysis of subdivided callus cultures revealed spatial segregation of chromosomal variability. Cytogenetic analyses were performed to determine the expression of this in vitro chromosomal variability in corresponding regenerated plant tissues. A complete loss of polyploidy and a decrease in aneuploidy and chromosomal rearrangements were observed. Analyses of specific isozyme activities in regenerates suggested that a quantitative segregation of H. vulgare and H. jubatum genomes had occurred in tissue cultures of their interspecific hybrid. Possible uses of in vitro chromosomal variability for plant breeding and genetical studies are discussed.

Chromosomal variability in tissue cultures and regenerated plants of Hordeum