Autotetraploids of tomatillo were obtained following the method of Pandey (1957). Of the 4 colchiploids 1 was different from the rest having wrinkled leaves, stunted growth and being highly sterile. On cytological check it was desynaptic. Studies on the chromosome pairing in both the desynaptic and non-desynaptic tetrapolids revealed higher univalent frequency coupled with lower chiasma frequency and higher sterility in the desynaptic tetraploid. The inheritance of desynapsis could not be studied because of complete seed sterility. External environmental factors as a possible causative agency for the induction of desynapsis are ruled out. Whether desynapsis was due to colchicine induced mutation or physiological upset in the plant could not be ascertained.