Cleavage of rauscher murine leukemia virus pr 65g a g by the moloney murine leukemia virus proteolytic activity produces rauscher murine leukemia virus specific but not moloney murine leukemia virus specific 40000 dalton intermediate poly peptide
Yoshinaka, Y.; Luftig, R.B.
Journal of General Virology 54(1): 33-38
ISSN/ISBN: 0022-1317 Accession: 004963867
Although the Pr65gag precursor polyproteins of Moloney murine leukemia virus (M-MuLV) and of Rauscher murine leukemia virus (R-MuLV) have the same apparent MW by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), their initial 40,000 dalton intermediate cleavage products differ in MW, i.e., the M-MuLV product (Pr41.5gag) is 1500 daltons larger than the R-MuLV product (Pr40gag). In vitro cleavage of R-MuLV Pr65gag by the M-MuLV proteolytic activity gave rise to R-MuLV Pr40gag and not M-MuLV Pr41.5gag. This suggests that the specificity for cleavage of the MuLV Pr65gag is built into the substrate.