EurekaMag.com logo
+ Site Statistics
References:
54,215,046
Abstracts:
30,230,908
PMIDs:
28,215,208
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Cloning of genes involved in pathogenicity of xanthomonas campestris pathovar campestris using the broad host range cosmid plafr 1



Cloning of genes involved in pathogenicity of xanthomonas campestris pathovar campestris using the broad host range cosmid plafr 1



EMBO (European Molecular Biology Organization) Journal 3(13): 3323-3328



A genomic library was prepared in Escherichia coli from DNA of wild-type X. campestris pv. campestris (etiological agent of crucifer black rot), partially digested with endonuclease EcoRI, using the mobilizable broad host range cosmid vector pLAFR1. Recombinant plasmids contained inserts ranging in size from 19.1 to 32.3 kb [kilobase] (mean 26.6). Certain of the clones complemented E. coli auxotrophic markers. Using the narrow host range plasmid pRK2013 as a helper the pooled recombinant plasmids were transferred conjugally to X. campestris campestris mutants, and clones were identified which restored yellow pigmentation to white mutants, prototrophy to amino acid auxotrophs and pathogenicity towards turnip plants to 2 nonpathogenic mutants. The lesion in 1 mutant (8288, complemented by the plasmid pIJ3000) is unknown. Mutant 8237 is defective in production of extracellular protease and polygalacturonate lyase and restoration of pathogenicity by complementation with the plasmid pIJ3000 concomitantly restored both enzyme levels to wild-type values.

(PDF emailed within 1 workday: $29.90)

Accession: 004977256

Download citation: RISBibTeXText



Related references

Cloning of genes involved in pathogenicity of Xanthomonas campestris pv. campestris using the broad host range cosmid pLAFR1. Embo Journal 3(13): 3323-3328, 1984

Broad host range cosmid plafr1 and non mucoid mutant xcp20 provide a suitable vector host system for cloning genes in xanthomonas campestris pathovar campestris. Chinese Journal of Microbiology & Immunology (Taipei) 21(1): 40-49, 1988

Broad host range cosmid pLAFR1 and non-mucoid mutant XCP20 provide a suitable vector-host system for cloning genes in Xanthomonas campestris pv. campestris. Zhonghua Minguo Wei Sheng Wu Ji Mian Yi Xue Za Zhi 21(1): 40-49, 1988

A multipurpose broad host range cloning vector and its use to characterise an extracellular protease gene of Xanthomonas campestris pathovar campestris. Molecular and General Genetics 220(3): 433-440, 1990

Xanthomonas campestris pathovar oryzae has dna sequences containing genes isofunctional with xanthomonas campestris pathovar campestris genes required for pathogenicity. Physiological & Molecular Plant Pathology 36(1): 73-87, 1990

Molecular cloning of protease genes from xanthomonas campestris pathovar campestris expression in escherichia coli and role in pathogenicity. Molecular & General Genetics 210(3): 443-448, 1987

Molecular cloning of protease and cellulase genes from Xanthomonas campestris pathovar campestris: Role of the gene products in pathogenicity. Molecular genetics of plant microbe interactions 1988: proceedings of the 4th International Symposium on Molecular Genetics of Plant Microbe Interactions Acapulco Mexico May 15-20-1988 edited by Rafael Palacios: 268, 1988

Molecular cloning of genes involved in the production of the extracellular polysaccharide xanthan by xanthomonas campestris pathovar campestris. International Journal of Biological Macromolecules 8(6): 372-374, 1986

Cloning of genes involved in negative regulation of production of extracellular enzymes and polysaccharide of Xanthomonas campestris pathovar campestris. Molecular & General Genetics 222(1): 157-160, 1990

The rpfA gene of Xanthomonas campestris pathovar campestris, which is involved in the regulation of pathogenicity factor production, encodes an aconitase. Molecular Microbiology 28(5): 961-970, 1998

Evidence for clustered pathogenicity genes in xanthomonas campestris pathovar campestris. Molecular & General Genetics 199(2): 338-343, 1985

Cloning and analysis of a 35.3 kilobase dna region involved in exopolysaccharide production by xanthomonas campestris pathovar campestris. Journal of Bacteriology 172(5): 2804-2807, 1990