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Cloning the structural gene for diphtheria toxin from corynebacterium diphtheriae pw8 and expression of its deleted derivative in escherichia coli



Cloning the structural gene for diphtheria toxin from corynebacterium diphtheriae pw8 and expression of its deleted derivative in escherichia coli



Molekulyarnaya Genetika Mikrobiologiya i Virusologiya (11): 13-18



The coding region of diphtheria toxin gene from Corynebacterium diphtheriae PW8 was cloned in Escherichia coli on pUC19 plasmid. 5' end of the gene was deleted. The deleted gene codes for a toxin-like protein truncated for 36 N-terminal aminoacids. The modified gene was fused with 5' terminal part of the lacZ gene on pUC19 plasmid having kept the translational frame intact. The hybrid gene transcription depends on lacP promoter, Mr of the resulting toxoid is 54000.

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