Clover secondary metabolites 6. iso flavones and pterocarpans in red clover roots inoculated with nodule bacteria rhizobium trifolii

Popravko, S.A.; Sokolova, S.A.; Kononenko, G.P.

Bioorganicheskaya Khimiya 6(8): 1255-1264


ISSN/ISBN: 0360-4497
Accession: 004978372

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GLC-MS of Me3Si-ethers of isoflavone and pterocarpan total fractions showed that the roots of red clover seedlings grown in sterile conditions contained the group of isoflavones-formononetin (III), pseudobaptigenin (IV), biochanin A (VI), and kalikosine (V), but no pterocarpans-maackiain: (inermin) (II) or trifolirizin (I). The composition of this group of compounds in roots was unchanged after inoculation of seedlings with nodule bacteria (Rhizobium trifolii). The concentration of pterocarpan (II) in roots analyzed by GLC method exceeded that of isoflavones (III) and (IV) in equal-aged seedlings grown in field. Isoflavones genistein (VIII) and irilone (VII) which manifested in vitro high toxicity against fungi were present in roots at < 5 mg% dry matter. Comparative toxicity of compounds (I), (II), (IV)-(VIII) against Sclerotinia trifoliorum and Botrytis cinerea was assayed in vitro. The data on mass spectra of Me3Si-ethers of isoflavones and pterocarpans are discussed.