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Clustered immuno globulin g on human red blood cell membranes may promote human lymphocyte antibody dependent cell mediated cyto toxicity



Clustered immuno globulin g on human red blood cell membranes may promote human lymphocyte antibody dependent cell mediated cyto toxicity



Journal of Immunology 125(2): 501-507



Human K [antibody-dependent killer] lymphocytes carry out antibody-dependent cell-mediated cytotoxicity (ADCC) toward a variety of target cell types excluding allogeneic erythrocytes (RBC). Evidence was presented recently which indicates that certain human RBC-antibody combinations are actually quite susceptible to K lymphocyte ADCC. These targets, which include papain-treated CD.hivin.e/.hivin.cDE RBC or -D-/-D- cells each sensitized with small amounts of anti-D IgG and normal CD.hivin.e/.hivin.cDE RBC sensitized with high titer anti-D or anti-CD IgG sera, possess as a common feature clustered surface IgG. This study examined K lymphocyte ADCC to these same targets and simultaneously measured the amount of target bound IgG by 125I-staphylococcal protein A (SPA) assay. CD.hivin.e/.hivin.cDE erythrocytes, which have dispersed D antigens, were coated with .apprx. 9000, 5000 and 1500 anti D IgG/cell but were lysed poorly, if at all, by lymphocytes (6, 1 and 0% 51Cr release, respectively). These same targets pretreated with papain to cluster D antigens were quite sensitive to lymphocyte ADCC when coated with the same numbers of IgG molecules (81, 72 and 34% 51Cr release, respectively). -D-/-D- RBC, which possess clustered anti-D IgG, were lysed significantly by lymphocytes (42% 51Cr release with 8400 IgG/cell and 28% 51Cr release with 5400 IgG/cell). Loss of sialic acid and decrease in surface charge from enzyme-treated RBC targets correlated roughly with enhanced susceptibility to lymphocyte ADCC for some enzymes (papain and neuraminidase) but not for others (trypsin). Osmotic fragility of enzyme-treated cells was normal and does not account for their sensitivity to lysis. Apparently, the spatial arrangement of IgG on the target cell surface may play an important role in determining whether K lymphocytes express Fc receptor activity in terms of binding and lysing antibody-coated RBC.

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Accession: 004978581

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Clustered IgG on human red blood cell membranes may promote human lymphocyte antibody-dependent cell-mediated cytotoxicity. Journal of Immunology 125(2): 501-507, 1980

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