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Cold sensitive cell division cycle mutants of yeast saccharomyces cerevisiae isolation properties and pseudoreversion studies



Cold sensitive cell division cycle mutants of yeast saccharomyces cerevisiae isolation properties and pseudoreversion studies



Genetics 100(4): 547-564



Independent recessive cold-sensitive cell-division-cycle (cdc) mutants (18) of S. cerevisiae in 9 complementation groups were isolated. Terminal phenotypes exhibited include medial nuclear division, cytokinesis, and a previously undescribed terminal phenotype consisting of cells with a single small bud and an undivided nucleus. Four of the cold-sensitive mutants were alleles of CDC11, while the remaining mutants defined at least 6 new cell-division-cycle genes: CDC44, CDC45, CDC48, CDC49, CDC50 and CDC51. Spontaneous revertants from cold-sensitivity of 4 of the medial nuclear division cs cdc mutants were screened for simultaneous acquisition of a temperature-sensitive phenotype. The temperature-sensitive revertants of 4 different cs cdc mutants carried single new mutations, called Sup/Ts to denote their dual phenotype: suppression of the cold-sensitivity and concomitant conditional lethality at 37.degree. Many of the Sup/Ts mutations exhibited a cell-division-cycle terminal phenotype at the high temperature, and they defined 2 new cdc genes (CDC46 and CDC47). Two cold-sensitive medial nuclear division cdc mutants representing two different cdc genes were suppressed by different Sup/Ts alleles of another gene which also bears a medial nuclear division function (CDC46). In addition, the cold-sensitive medial nuclear division cdc mutant csH80 was suppressed by a Sup/Ts mutation yielding an unbudded terminal phenotype with an undivided nucleus at the high temperature. This mutation was an allele of CDC32. A pattern was suggested of interaction among cdc gene products. Possibly, cdc gene proteins might act in the cell cycle as complex specific functional assemblies.

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