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Column selection of antigenic variants from tumors yac moloney lymphoma variants with reduced antigen expression



Column selection of antigenic variants from tumors yac moloney lymphoma variants with reduced antigen expression



Immunogenetics 9(4-5): 465-476



Serial pretreatment of YAC lymphoma cells with anti H-2a serum and anti-Ig [immunoglobulin] column selection to remove cells with high surface H-2 concentration, followed by intermittent transfer into mice has led to the establishment of 2 variant sublines with low H-2a antigen expression and only a slightly reduced Moloney virus-determined cell surface antigen (MCSA) expression compared to the original YAC line. Both sublines had an increased number of chomosomes with a modal number of 62 and 57, as compared to 39 in YAC. In contrast to YAC, the variants were freely homotransplantable across the H-2 barrier. They resisted the rejection response of YAC-preimmunized semisyngeneic mice. Parallel selection against MCSA resulted in a variant with reduced MCSA, but unchanged H-2a expression and karyotype. This subline failed to grow across the allograft barrier and showed only slightly increased ability to grow in preimmunized semisyngeneic mice. H-2 expression may play an important role in the ability of antigenic tumor cells to be rejected in specifically preimmunized, genetically compatible recipients.

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Accession: 004986697

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DOI: 10.1007/bf01570439


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