+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Combined immuno fluorescence and transmission electron microscopic studies of sequential intestinal samples from turkey embryos and poults infected with turkey enteritis coronavirus



Combined immuno fluorescence and transmission electron microscopic studies of sequential intestinal samples from turkey embryos and poults infected with turkey enteritis coronavirus



American Journal of Veterinary Research 39(8): 1348-1354



Turkey embryos (24 days old) were inoculated with turkey enteritis coronavirus and intestinal tissues were examined by immunofluorescence and transmission electron microscopy at 6, 12, 24, 48, 72, 96, 120, 168 and 336 h after inoculation. Coronaviral antigens were first detected at 12 h in a few cells located at the base of villi. By 24 h, the villi were markedly shortened and immunofluorescent cells were observed over the entire villi and occasionally in the crypts. At 48 h, antigen containing cells were found in the crypts and covering the upper half of the villi. Epithelial cell sloughing was observed at 72 and 96 h, at which time coronaviral antigens were found in the sloughing cells, in clusters of cells near the base of villi and in some crypt epithelial cells. In subsequent tissue samples, the villi returned to normal, but specific fluorescence persisted in small groups of cells randomly located on the villi and in the crypts. Ultrastructural alterations were confined to the epithelial cells and consisted of loss of microvilli, disruption of the terminal web region, abnormalities in mitochondrial size and morphology, dilation of cisternae in the endoplasmic reticular system, substantial increases in the amount of intracellular lipid and excessive sloughing of epithelial cells from the villi. The severity of these ultrastructural lesions did not correlate with clinical signs or mortality. Coronavirus particles were detectable by transmission electron microscopy from 24-96 h after inoculation. They were generally spherical with a mean diameter of 100-110 nm and had a central electron lucent matrix surrounded by an inner band of darkly staining subunits and an outer envelope with no distinguishing features. Virus replication occurred in the cytoplasm of infected cells by budding into the cisternae of the endoplasmic reticular system. Plasma membrane budding was not observed.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 004988354

Download citation: RISBibTeXText

PMID: 211883


Related references

Characterization of turkey coronavirus from turkey poults with acute enteritis. Veterinary Microbiology 84(1/2): 179-186, 2002

Characterization of turkey coronavirus from turkey poults with acute enteritis. Veterinary Microbiology 84(1-2): 179-186, 2002

Humoral and cellular immune responses in turkey poults infected with turkey coronavirus. Poultry Science 80(10): 1416-1424, 2001

Specific mucosal IgA immunity in turkey poults infected with turkey coronavirus. Veterinary Immunology and Immunopathology 88(1/2): 57-64, 2002

Pathology and tissue distribution of turkey coronavirus in experimentally infected chicks and turkey poults. Journal of Comparative Pathology 143(1): 8, 2010

Infection with a pathogenic turkey coronavirus isolate negatively affects growth performance and intestinal morphology of young turkey poults in Canada. Avian Pathology 38(4): 279-286, 2009

Diagnosis of turkey viral enteric diseases by electron microscopy and identification of coronavirus in a case of turkey enteritis. Large Animals Review 8(6): 91-92, 2002

A sequential histopathologic and immunocytochemical study of chickens, turkey poults, and broiler breeders experimentally infected with turkey rhinotracheitis virus. Avian Diseases 39(4): 887-896, 1995

Combined feeds for turkey poults. 1. Effect of DL-methionine and L. lysine on growth and meat quality of turkey poults. Zhivotnovudni Nauki. 8: 8, 25-30, 1971

Multiplex Real-Time Reverse Transcription-Polymerase Chain Reaction for the Detection of Three Viruses Associated with Poult Enteritis Complex: Turkey Astrovirus, Turkey Coronavirus, and Turkey Reovirus. Avian Diseases 49(1): 86-91, 2005

Multiplex real-time reverse transcription-polymerase chain reaction for the detection of three viruses associated with poult enteritis complex: turkey astrovirus, turkey coronavirus, and turkey reovirus. Avian Diseases 49(1): 86-91, 2005

Ultrastructural changes in the intestines of turkey poults and embryos affected with transmissible enteritis. Journal of Comparative Pathology 82(2): 187-192, 1972

Histopathology of fasting and bluecomb disease in turkey poults and embryos experimentally infected with bluecomb disease coronavirus. Avian Diseases 20(4): 631-640, 1976

The effect of immunosuppression on protective immunity of turkey poults against infection with turkey coronavirus. Comparative Immunology, Microbiology and Infectious Diseases 25(2): 127-138, 2002

Detection of turkey coronavirus in commercial turkey poults in Brazil. Avian Pathology 36(1): 29-33, 2007