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Comparative studies on nucleolar organizer regions of normal and leukemia cells of the rat by silver nucleolar organizer regions staining and in situ hybridization methods



Comparative studies on nucleolar organizer regions of normal and leukemia cells of the rat by silver nucleolar organizer regions staining and in situ hybridization methods



Medical Journal of Kobe University 43(4): 41-48



Nucleolar organizer regions (NOR) of normal rat bone marrow cells, fibroblasts and 9 erythroblastic leukemia cell lines were studied by Ag-NOR staining and in-situ hybridization methods. Normal bone marrow cells in vivo and fibroblasts in vitro had an average of 5.6 and 5.8 Ag-positive NOR (Ag[+]NOR) for 6 NOR per metaphase, respectively. Growth stimuli given 24 h before harvesting of bone marrow cells slightly increased the incidence of Ag(+)NOR. Ag(+)NOR in normal bone marrow cells in vitro decreased in number as time passed. Leukemia cells had a lower number of Ag(+)NOR than the normal bone marow cells and fibroblasts. The distribution of Ag(+)NOR among NOR in leukemia cells was abnormal and different from case to case. Distribution of Ag(+)NOR was different from that of ribosomal cistrons shown by the in-situ hybridization method. Interphase nucleoli were much more heavily labeled with Ag-NOR grains. The abnormal increase and distribution pattern of ribosomal DNA and abnormal pattern of their activity in leukemia cells were discussed.

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Accession: 004999194

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