Section 5
Chapter 5,000

Comparative study of 3 tests dye test indirect hem agglutination test latex agglutination test for the detection of antibodies to toxoplasma gondii in human sera

Balfour, A.H.; Fleck, D.G.; Hughes, H.P.A.; Sharp, D.

Journal of Clinical Pathology 35(2): 228-232


ISSN/ISBN: 0021-9746
Accession: 004999932

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An evaluation was made of a commercial latex agglutination test, Toxotest-MT (TMT) (Eiken, Japan), for the detection of antibodies to T. gondii in human sera. In qualitative studies, 878 sera were examined in both the TMT and dye test (DT) and 96.6% agreement was found. In quantitative studies 339 sera were titrated in the TMT and DT, with 337 of these sera also titrated in the indirect hemagglutination test (IHAT). Agreement between the DT and TMT was best, 78% of the sera showing titers within .+-. 1 dilution in the 2 tests. The IHAT and TMT gave 66% agreement, while the DT and IHAT showed least agreement, 40.9%. Apparently, the pattern of antigenic determinants to which antibody levels are measured are different in the 3 test systems. The TMT is a better substitute for the DT than the IHAT.

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