Comparative study of d xylose conversion to ethanol by immobilized growing or non growing cells of the yeast pachysolen tannophilus

Amin, G.; Khallafalla, G.; Doelle, H.W.

Applied Microbiology and Biotechnology 27(4): 325-332

1988


ISSN/ISBN: 0175-7598
Accession: 005000243

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Abstract
The direct conversion of D-xylose to ethanol was investigated using immobilized growing and non-growing cells of the yeast Pachysolen tannophilus. Both preparations produced ethanol from D-xylose, however the D-xylose conversion to ethanol was much better with immobilized growing cells. Ethanol concentration up to 22.9 g/l and ethanol yield of 0.351 g/g of D-xylose were obtained in batch fermentation by immobilized growing cells whereas only 17.0 g/l and 0.308 g/g of D-xylose were obtained by immobilized nongrowing cells were necessary for the long-term operation, since a steady state ethanol concentration of 17.7 g/l was maintained for only one week by immobilized non-growing cell reactor. With continuous systems, immobilized growing cells were necessary for the long-term operation, since a steady state ethanol concentration of 17.7 g/l was maintained for only one week by immobilized non-growing cell reactor. With simultaneous control of aeration rate and concentrations of nitrogen sources in feed medium, immobilized growing cells of P. tannophilus showed excellent performance. At a residence time of 25 h, the immobilized cell reactor produced 26.9 g/l of ethanol from 65 g/l of D-xylose in feed medium.