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Comparison of co agglutination latex agglutination and enzyme linked immuno sorbent assay elisa for detection of haemophilus influenzae type b infection



Comparison of co agglutination latex agglutination and enzyme linked immuno sorbent assay elisa for detection of haemophilus influenzae type b infection



Diagnostic Microbiology & Infectious Disease 1(4): 317-322



Two commercially available kits were compared to an ELISA for detection of H. influenzae type b antigen polyribophosphate in clinical specimens with infections proven by culture. Methods employed by the kits were latex agglutination (LA) and staphylococcal coagglutination (COA). The COA kit detects H. influenzae type b and types a and c-f with a polyvalent antiserum; the ELISA assay and the LA kit detect only type b. Specimens (41 spinal fluid, 35 urine, 25 serum and 38 sputum) were tested. All spinal fluid samples positive by culture were positive by all 3 procedures. Of urine specimens from patients with a variety of H. influenzae type b infections, 13 of 15 were positive by ELISA, 8 of 15 by COA and 8 of 14 by LA. Of serum samples collected from the same patients at various times during their illness, 8 of 15 were positive by ELISA, 6 of 15 by COA and 10 of 15 by LA. Of sputum samples positive by culture for H. influenzae type b, 14 of 17 were positive by ELISA, 9 of 17 by COA and 4 of 16 by LA. The ability to detect additional serotypes of H. influenzae was shown by the COA kit, which detected H. influenzae type a in spinal fluid from a patient with type a meningitis proven by culture.

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