+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Comparison of four microtiter plate enzyme immunoassays for milk progesterone determination in cows

Comparison of four microtiter plate enzyme immunoassays for milk progesterone determination in cows

Zuchthygiene (Berlin) 22(6): 247-252

Four enzyme immunoassay (EIA)-microtitre plate-tests for progesterone determination in cows' milk were tested in a comparative trial. These were "Epignost"/Noctech, "Ovucheck"/Cambridge Veterinary Sciences, "Enzygnost"/Hoest, "Schoonoord-Test"/Zeist. Milk from 22 cows each with low, medium or high progesterone level, respectively, was utilized. Each sample was tested in duplicate on 3 plates from each manufacturer. Absolute values between assay differed considerably, though correlations were high (average 0.94). In order to make comparisions, between results obtained with different assays regressions with quadratic and cubic functions were established. The most important component of variance was "sample" (between 72% and 86%). The remaining variance was essentially shared between individual plate and replicate. The time required for a determination varied between 35 to 40 min ("Enzygnost") and 120 min ("Schoonoord"). In spite of certain differences with regard to precision and time required each of the 4 tests appears to be suited for routine milk progesterone determinations. The EIA microtitre plate-test, being fast, simple and not costly will gain in popularity especially since further simplification and cost reduction is to be expected.

(PDF emailed within 1 workday: $29.90)

Accession: 005007970

Download citation: RISBibTeXText

Related references

The influence of heterology enzyme label and assay conditions on the sensitivity of microtiter plate enzyme immunoassays for progesterone in milk. Journal of Steroid Biochemistry 33(3): 433-438, 1989

A comparison of alkaline phosphatase beta galactosidase penicillinase and peroxidase used as labels for progesterone determination in milk by heterologous microtiter plate enzyme immunoassay. Journal of Steroid Biochemistry 33(3): 423-432, 1989

Development of a microtiter plate enzyme immunoassay for progesterone determination in pig blood plasma and its validation and comparison to radioimmunological methods for progesterone determination. Zuchthygiene (Berlin) 20(4): 184-191, 1985

Measurement of progesterone in skim milk of cows by a microtiter plate enzyme immunoassay using a double antibody method and its application to pregnancy diagnosis. Japanese Journal of Animal Reproduction 34(3): 167-173, 1988

Determination of milk progesterone concentration by means of different microtitre-plate enzyme immunoassays. Zuchthygiene 22(6): 247-252, 1987

Comparison of nonradioactive microtiter plate enzyme immunoassays for the sensitive detection of fentanyl. Forensic Science International 113(1-3): 353-357, September 11th, 2000

A comparison of two enzyme immunoassays for the determination of milk and serum progesterone in horses and cattle and serum progesterone in pigs. Zuchthygiene 21(5): 241-246, 1986

Use of microtiter plate enzyme immunoassay kit for direct determination of progesterone in bovine blood plasma. Japanese Journal of Animal Reproduction 33(3): 134-139, 1987

Correlation between homologous and heterologous enzyme immunoassays for progesterone determinations in milk from cows. Revista Espanola de Fisiologia 46(3): 303-308, 1990

Comparison of two enzyme immunoassays for progesterone in milk and blood serum from mares and cows and blood serum from sows. Zuchthygiene (Berlin) 21(5): 241-246, 1986

Determination of progesterone in cows milk comparison of the radioimmunoassay performed on fat extract with the enzyme immunoassay performed on unextracted milk and assessment of the reliability of the enzyme immunoassay. Zuchthygiene (Berlin) 20(1): 34-40, 1985

Comparison of 2 enzyme immunoassays and a radioimmunoassay for measurement of progesterone concentrations in bovine plasma, skim milk, and whole milk. Canadian Journal of Veterinary Research 72(1): 32-36, 2008

Comparison of a lateral flow milk progesterone test with enzyme immunoassay as an aid for reproductive status determination in cows. Veterinary Record 178(11): 260-260, 2016