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Comparison of four microtiter plate enzyme immunoassays for milk progesterone determination in cows



Comparison of four microtiter plate enzyme immunoassays for milk progesterone determination in cows



Zuchthygiene (Berlin) 22(6): 247-252



Four enzyme immunoassay (EIA)-microtitre plate-tests for progesterone determination in cows' milk were tested in a comparative trial. These were "Epignost"/Noctech, "Ovucheck"/Cambridge Veterinary Sciences, "Enzygnost"/Hoest, "Schoonoord-Test"/Zeist. Milk from 22 cows each with low, medium or high progesterone level, respectively, was utilized. Each sample was tested in duplicate on 3 plates from each manufacturer. Absolute values between assay differed considerably, though correlations were high (average 0.94). In order to make comparisions, between results obtained with different assays regressions with quadratic and cubic functions were established. The most important component of variance was "sample" (between 72% and 86%). The remaining variance was essentially shared between individual plate and replicate. The time required for a determination varied between 35 to 40 min ("Enzygnost") and 120 min ("Schoonoord"). In spite of certain differences with regard to precision and time required each of the 4 tests appears to be suited for routine milk progesterone determinations. The EIA microtitre plate-test, being fast, simple and not costly will gain in popularity especially since further simplification and cost reduction is to be expected.

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Accession: 005007970

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