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Comparison of nitrogen 15 labeled glycine aspartate valine and leucine for measurement of whole body protein turnover



Comparison of nitrogen 15 labeled glycine aspartate valine and leucine for measurement of whole body protein turnover



Clinical Science 57(3): 281-284



Whole-body protein turnover was measured in rats by constant infusion of 15N-labeled glycine, aspartate, valine and leucine and measuring hepatic and renal urea and NH3 N. Values obtained with [15N]glycine were comparable with those reported with methods based on different assumptions. [15N]Aspartate gave rise to an increased enrichment of urea and NH3, thus to lower protein turnover rates. [15N]Valine and [15N]leucine gave low enrichments of nitrogenous end-products, hence to high protein-turnover rates. All 15N-labeled amino acids are not equally suitable for measuring whole-body protein turnover by the end-product method. The relative amounts of 15N going to the end-products can be predicted from the known individual metabolism of aspartate and the branched-chain amino acids.

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Accession: 005010384

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