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Chapter 5,018

Compatibility in prunus avium prunus cerasus graftings during the initial phase 3. isoelectrofocusing of proteins peroxidases and acid phosphatases during union formation

Schmid, P.P.S.; Feucht, W.

Journal of Horticultural Science 60(3): 311-318

1985


Accession: 005017495

The sweet cherry cv. Sam (P. avium) was splice-grafted onto rootstocks from F12/1 (P. avium) and from P. cerasus selections Cer W10, W11 and W13. Five trees from each graft combination were harvested weekly for 8 wk after grafting. From these trees 3 sectors along the graft union were examined, below, at and above the union. The phloem from 2 consecutive weeks was combined and the corresponding peroxidases, acid phosphatases and proteins were isoelectrofocused on ultra-thin polyacrylamide gels. Initially, during weeks 1 and 2, only the peroxidases from the union itself showed some response to grafting as the bands exhibited diffuse patterns. This reaction was dramatically enhanced during weeks 3 and 4. This was followed in weeks 5 and 6 by a strong diminution of general banding intensity in all 3 sectors. From weeks 3 to 6 the combination Sam/Cer W11, which was somewhat incompatible in this experiment, reacted most intensely. In weeks 7 and 8 a restoration of peroxidases took place. Another feature was observed with respect to the acid phosphatases; they reached maximal intensity directly after grafting that is by weeks 1 and 2. Despite the fact that they did not show any clear relationships, the following 4 wk were characterized by the changing intensities of the bands. Weeks 7 and 8, however, showed somewhat stable and medium levels of phosphatases in all the grafts and graft sectors which were studied. The proteins varied least throughout the experiment, although a marked maximum of band intensity was reached during weeks 3 and 4.

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