Competitive enzyme immunoassay for human chorionic somatomammotropin using the avidin-biotin system
Rappuoli, R.; Leoncini, P.; Tarli, P.; Neri, P.
Analytical Biochemistry 118(1): 168-172
ISSN/ISBN: 0003-2697 PMID: 7039407 DOI: 10.1016/0003-2697(81)90174-3
Human chorionic somatomammotropin (human placental lactogen, HCS) is determined by an enzyme immunoassay where HCS competes with biotin-labeled HCS for insolubilized anti-HCS antibodies. Enzyme-labeled avidin is then used to reveal the amount of bound HCS. The system proves to be sensitive (1 ng/ml of HCS can be detected) and results agree with radioimmunoassay determinations (r = 0.979). Kinetics of the avidin-biotin reaction and coating of polystyrene wells are also investigated.