Complexes of thrombin with secreted platelet proteins

Miller, J.J.; Browne, P.C.; Detwiler, T.C.

Biochemical and Biophysical Research Communications 151(1): 9-15


ISSN/ISBN: 0006-291X
PMID: 3126741
DOI: 10.1016/0006-291x(88)90552-9
Accession: 005021625

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A labeled 77-kDa complex formed when 125I-thrombin was added to platelet suspensions or to the supernatant solution of ionophore-activated platelets. Prostacyclin inhibited complex formation with whole platelets but not with the supernatal solution of ionophore-activated platelets. This is evidence that the complex formed with a factor secreted from activated platelets. Smaller complexes of 70 and 58 kDa formed between labeled thrombin and lysed platelets. The 77-kDa complex was necessary for the formation of a thrombin-thrombospondin complex.