Home
  >  
Section 6
  >  
Chapter 5,034

Conformation and aggregation of a polysaccharide in solution as transported in golgi vesicles and in an extracellular matrix

Trachtenberg, S.

Journal of Ultrastructure and Molecular Structure Research 97(1-3): 89-102

1986


ISSN/ISBN: 0889-1605
DOI: 10.1016/s0889-1605(86)80009-x
Accession: 005033049

Download citation:  
Text
  |  
BibTeX
  |  
RIS

The structure of the extracellular matrix polysaccharide (ECMP) of Opuntia fiscus-indica was studied by transmission and scanning transmission electron microscopy and image analysis. In isolated form, in solution, the ECMP appears long and unbranched. Linear mass measurements indicate that, in solution, aggregation of the polysaccharide chains into bundles occurs. The gelling properties at various pHs and the structure of the gels' junction zones were studied by using Alcian blue 8GX as an artificial gelling agent. The ECMP-Alcian blue junction zones are electrostatic in nature. They are constructed from parallel ECMP strands cross-bridged by Alcian blue molecules with lateral and axial repeats of 30 and 35 .ANG., respectively. Similar structures can be formed with polygalacturonic, alginic, and hyaluronic acids and heparin. Parallel studies on the polysaccharides morphology, as transported in Golgi vesicles and in the extracellular matrix, were carried out on thin sections stained for polysaccharides.

PDF emailed within 0-6 h: $19.90